Search results for "Cell model"
showing 10 items of 15 documents
7-ketocholesterol and 7β-hydroxycholesterol: in vitro and animal models used to characterize their activities and to identify molecules preventing th…
2020
International audience; Oxysterols are molecules derived by the oxidation of cholesterol and can be formed either by auto-oxidation, enzymatically or by both processes. Among the oxysterols formed by auto-oxidation, 7-ketocholesterol and 7beta-hydroxycholesterol are the main forms generated. These oxysterols, formed endogenously and brought in large quantities by certain foods, have major cytotoxic properties. They are powerful inducers of oxidative stress, inducing dysfunction of organelles (mitochondria, lysosomes and peroxisomes) that can cause cell death. These molecules are often identified in increased amounts in common pathological states such as cardiovascular diseases, certain eye …
Fluorinated and pegylated polyaspartamide derivatives to increase solubility and efficacy of Flutamide
2012
New fluorinated amphiphilic copolymers based on a biocompatible polyaspartamide have been prepared in order to obtain polymeric micelles useful for delivering anticancer drugs. In particular, α,β-poly(N-2-hydroxyethyl)-d,l-aspartamide (PHEA) has been derivatized with polyethylene glycol (PEG(2000)) and ethylendiamine (EDA). Both these portions form the hydrophilic part of the copolymer, while the hydrophobic moiety is given by 1,2,4-oxadiazoles: 5-pentafluorophenyl-3-perfluoroheptyl-1,2,4-oxadiazole (PPOX) or 3-carboxyethyl-5-pentadecafluoroheptyl-1,2,4-oxadiazole (CPOX). Copolymers named PHEA-PEG(2000)-EDA-PPOX and PHEA-PEG(2000)-EDA-CPOX have been prepared with various degrees of derivati…
Automated untargeted stable isotope assisted lipidomics of liver cells on high glucose shows alteration of sphingolipid kinetics
2020
Abstract Untargeted lipidomics is a powerful tool to discover new biomarkers and to understand the physiology and pathology of lipids. The use of stable isotopes as tracers to investigate the kinetics of lipids is another tool able to supply dynamic information on lipid synthesis and catabolism. Coupling the two methodology is then very appealing in the study of lipid metabolism. The main issue to face is to perform thousands of calculations in order to obtain kinetic parameters starting from the MS raw data. An automated computerized routine able to do accomplish such task is presented in this paper. We analyzed the lipid kinetics of palmitic acid (PA) in hepatoma liver cells cultured in v…
Cadmium-Associated Molecular Signatures in Cancer Cell Models
2021
Simple Summary The exposure of cancer cells to cadmium compounds may be associated with the acceleration of tumor progression. It is known that cadmium is a transcriptional regulator, and the study of differentially expressed genes has enabled the identification and classification of cadmium-associated molecular signatures as useful biomarkers that are potentially transferable to clinical research. This review recapitulates the studies that report the detection of such signatures in breast, gastric, colon, liver, lung, and nasopharyngeal tumor cell models, as specifically demonstrated by individual gene or whole genome expression profiling. Abstract The exposure of cancer cells to cadmium a…
The Industrial Applicability of PEA Space Charge Measurements, for Performance Optimization of HVDC Power Cables
2019
Cable manufacturing industries are constantly trying to improve the electrical performance of power cables. During the years, it was found that one of the most relevant degradation factors influencing the cable lifetime is the presence of space charge in the insulation layer. To detect the accumulated charge, the pulsed electro-acoustic (PEA) method is the most used technique. Despite the wide use of the PEA cell, several issues are still present. In particular, the PEA output signal is strongly disturbed by the acoustic waves reflections within the PEA cell. This causes the distortion of the output signal and therefore the misinterpretation of the charge profiles. This, in turn, may result…
The metabolic responses to electrical pulse-induced contractions in C2C12 myotubes are greater in high glucose medium
2021
Osaa liikunnan terveyshyötyjen mekanismeista on vaikeaa tutkia ihmisillä tai koe-eläimillä. Viljeltyjen C2C12 myotuubien sähköpulssistimulaatiolla (EPS, engl. electrical pulse stimulation) voidaan mallintaa lihassupistuksia ja liikuntaa in vitro –olosuhteissa. EPS:n vaikutusta C2C12 myotuubien aineenvaihduntaan ei kuitenkaan tunneta tarkasti. Lisäksi C2C12 myotuubeja viljellään yleensä korkeassa ei-fysiologisessa 25 mM glukoosipitoisuudessa, mikä voi vaikuttaa EPS-vasteisiin. Tässä tutkimuksessa C2C12 myotuubeja sähköpulssistimuloitiin 24 tunnin ajan sekä matalan 5.5 mM (MG) että korkean 25 mM (KG) glukoosipitoisuuden olosuhteissa, ja aineenvaihduntatuotteiden konsentraatiot analysoitiin se…
Proteomic characterization of the mucosal pellicle formed in vitro on a cellular model of oral epithelium
2019
International audience
A mucosal pellicle modifies the physical properties of epithelial
2017
International audience
Effect of caseinophosphopeptides added to fruit beverages upon ferritin synthesis in Caco-2 cells
2010
Ferritin synthesis was evaluated in iron sulphate solutions and in fruit beverages supplemented with iron, with/without zinc in the presence of caseinophosphopeptides (CPPs), based on a system combining simulated gastrointestinal digestion and the Caco-2 cell model. In iron sulphate solutions with/without zinc, the addition of CPPs had no effect upon ferritin synthesis. When CPPs were added to the bioaccessible fraction of fruit beverages supplemented with iron, ferritin synthesis increased in the same proportion as with the addition of milk. The addition of zinc to fruit beverages supplemented with iron and to iron sulphate solutions decreased ferritin synthesis. Nevertheless, in fruit bev…
Customised in vitro model to detect human metabolism-dependent idiosyncratic drug-induced liver injury
2017
Drug-induced liver injury (DILI) has a considerable impact on human health and is a major challenge in drug safety assessments. DILI is a frequent cause of liver injury and a leading reason for post-approval drug regulatory actions. Considerable variations in the expression levels of both cytochrome P450 (CYP) and conjugating enzymes have been described in humans, which could be responsible for increased susceptibility to DILI in some individuals. We herein explored the feasibility of the combined use of HepG2 cells co-transduced with multiple adenoviruses that encode drug-metabolising enzymes, and a high-content screening assay to evaluate metabolism-dependent drug toxicity and to identify…